Review



pegfp n1 vector between sali  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    TaKaRa pegfp n1 vector between sali
    Pegfp N1 Vector Between Sali, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/Sal+I/pmc11494317__pnas__2402674121__sapp-233-9-13
    Average 96 stars, based on 3342 article reviews
    pegfp n1 vector between sali - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. Plasmid encoding ORMDL3 was cloned in pCMV-HA/pCMV-Myc/Pcmv-flag vector, and ORMDL3 truncations ORMDL3(1-42), ORMDL3(43-82), ORMDL3(83-118) and ORMDL3(119-153) were constructed into the pEGFP-N1 vector (CLONTECH Laboratories). ..

    Article Title: DNA polymerase Lambda is anchored within the NHEJ synaptic complex via Ku70/80
    Article Snippet: .. The gap-filling reporter substrate was assembled into the pEGFP-N1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see for a detailed description). .. The Cpf1 and guide RNA co-expression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker ; Addgene plasmid # 74042 ; http://n2t.net/addgene:74042 ; RRID:Addgene_74042).

    Article Title: A comprehensive study on the multifunctional properties of galectin-4 in red-lip mullet (Planiliza haematocheilus): Insights into molecular interactions, antimicrobial defense, and cell proliferation.
    Article Snippet: Galectin-4 belongs to the galactoside-binding protein family and is a type of tandem repeat galectin.. Despite previous studies indicating its importance in fish immunology, a comprehensive investigation is necessary to fully understand its role in immunomodulatory functions and cellular dynamics.. Therefore, this study aimed to explore the immunomodulatory functions of galectin-4 with a particular focus on its antimicrobial and cellular proliferative properties.

    Article Title: PLK2 disrupts autophagic flux to promote SNCA/α-synuclein pathology
    Article Snippet: Then, the cells were labeled with an Alexa Fluor secondary antibody (Invitrogen, A10037; 1:500), and images were captured via a fluorescence microscope (Olympus, Japan) and a confocal microscope (Nikon, Japan). .. EGFP-DCTN1 was generated by excising full-length DCTN1 cDNA from myc-DCTN1 [ ] and inserting it into the pEGFP-N1 vector (Clontech, 6085–1) at the HindIII and EcoRI sites. .. Point mutants of EGFP-SNCA [ ], Flag-PLK2 (Youbio, G121393) and EGFP-DCTN1 were generated via site-directed mutagenesis via a MutanBEST kit (Takara, R401) with the primers listed in Table S1.

    Article Title: Targeted ubiquitination of Na V 1.8 reduces sensory neuronal excitability
    Article Snippet: .. Briefly, the complete coding sequence (688 a.a.) was subcloned in-frame into the NheI/BamHI sites of pEGFP-N1 vector (Clontech) to produce plasmid pCAP-1A-eGFP. .. Full-length Rat SCLT1 cDNA generated by double digestion of pCAP-1A-eGFP with NheI/AgeI was subcloned into the XbaI/AgeI sites of pcDNA3.1B (Invitrogen), and the native translation termination codon was restored by site directed mutagenesis using the QuickChange system (Stratagene, La Jolla, CA) to produce the vector pcDNA3-CAP-1A (Referred to as Sclt1 hereinafter).

    Article Title: TLR2 immunotherapy suppresses neuroinflammation, tau spread, and memory loss in rTg4510 mice.
    Article Snippet: .. Human TLR2 was subcloned into pEGFP-N1 vector (Clontech) and the mutants (ΔLBD, ΔDimer, D327W/F349L, P681H) were generated by site-directed mutagenesis. .. HEK-Blue Null1 and HEK-Blue hTLR2 cells (5.4 × 104 cells per well) were seeded into the 96-well plate containing reagents in a volume of 180 μl per well and incubated in HEK-Blue Detection medium (InvivoGen) at 37 ◦C for 16 h. After 20 h, secreted embryonic alkaline phosphatase (SEAP) levels were determined using a spectrophotometer at 620 nm.

    Article Title: Determination of metal ion transport rate of human ZIP4 using stable zinc isotopes.
    Article Snippet: The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. .. The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus. ..

    Article Title: PLK2 disrupts autophagic flux to promote SNCA/α-synuclein pathology.
    Article Snippet: .. EGFP-DCTN1 was generated by excising full-length DCTN1 cDNA from myc-DCTN1 [72] and inserting it into the pEGFP-N1 vector (Clontech, 6085-1) at the HindIII and EcoRI sites. .. Point mutants of EGFP-SNCA [84], Flag-PLK2 (Youbio, G121393) and EGFP-DCTN1 were generated via site-directed mutagenesis via a MutanBEST kit (Takara, R401) with the primers listed in Table S1.

    Clone Assay:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. Plasmid encoding ORMDL3 was cloned in pCMV-HA/pCMV-Myc/Pcmv-flag vector, and ORMDL3 truncations ORMDL3(1-42), ORMDL3(43-82), ORMDL3(83-118) and ORMDL3(119-153) were constructed into the pEGFP-N1 vector (CLONTECH Laboratories). ..

    Construct:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. Plasmid encoding ORMDL3 was cloned in pCMV-HA/pCMV-Myc/Pcmv-flag vector, and ORMDL3 truncations ORMDL3(1-42), ORMDL3(43-82), ORMDL3(83-118) and ORMDL3(119-153) were constructed into the pEGFP-N1 vector (CLONTECH Laboratories). ..

    Polymerase Chain Reaction:

    Article Title: DNA polymerase Lambda is anchored within the NHEJ synaptic complex via Ku70/80
    Article Snippet: .. The gap-filling reporter substrate was assembled into the pEGFP-N1 vector (Clontech) following sequential insertion of various PCR products, oligonucleotide linkers and synthetic DNA fragments (see for a detailed description). .. The Cpf1 and guide RNA co-expression plasmid used to cleave the reporter substrate was generated by inserting the pre-annealed gRNA-GF-F and gRNA-GF-R oligonucleotides into the Esp3I restriction sites of pTE4398 (a gift from Ervin Welker ; Addgene plasmid # 74042 ; http://n2t.net/addgene:74042 ; RRID:Addgene_74042).

    Sequencing:

    Article Title: A comprehensive study on the multifunctional properties of galectin-4 in red-lip mullet (Planiliza haematocheilus): Insights into molecular interactions, antimicrobial defense, and cell proliferation.
    Article Snippet: Galectin-4 belongs to the galactoside-binding protein family and is a type of tandem repeat galectin.. Despite previous studies indicating its importance in fish immunology, a comprehensive investigation is necessary to fully understand its role in immunomodulatory functions and cellular dynamics.. Therefore, this study aimed to explore the immunomodulatory functions of galectin-4 with a particular focus on its antimicrobial and cellular proliferative properties.

    Article Title: Targeted ubiquitination of Na V 1.8 reduces sensory neuronal excitability
    Article Snippet: .. Briefly, the complete coding sequence (688 a.a.) was subcloned in-frame into the NheI/BamHI sites of pEGFP-N1 vector (Clontech) to produce plasmid pCAP-1A-eGFP. .. Full-length Rat SCLT1 cDNA generated by double digestion of pCAP-1A-eGFP with NheI/AgeI was subcloned into the XbaI/AgeI sites of pcDNA3.1B (Invitrogen), and the native translation termination codon was restored by site directed mutagenesis using the QuickChange system (Stratagene, La Jolla, CA) to produce the vector pcDNA3-CAP-1A (Referred to as Sclt1 hereinafter).

    Article Title: Determination of metal ion transport rate of human ZIP4 using stable zinc isotopes.
    Article Snippet: The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. .. The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus. ..

    Generated:

    Article Title: PLK2 disrupts autophagic flux to promote SNCA/α-synuclein pathology
    Article Snippet: Then, the cells were labeled with an Alexa Fluor secondary antibody (Invitrogen, A10037; 1:500), and images were captured via a fluorescence microscope (Olympus, Japan) and a confocal microscope (Nikon, Japan). .. EGFP-DCTN1 was generated by excising full-length DCTN1 cDNA from myc-DCTN1 [ ] and inserting it into the pEGFP-N1 vector (Clontech, 6085–1) at the HindIII and EcoRI sites. .. Point mutants of EGFP-SNCA [ ], Flag-PLK2 (Youbio, G121393) and EGFP-DCTN1 were generated via site-directed mutagenesis via a MutanBEST kit (Takara, R401) with the primers listed in Table S1.

    Article Title: TLR2 immunotherapy suppresses neuroinflammation, tau spread, and memory loss in rTg4510 mice.
    Article Snippet: .. Human TLR2 was subcloned into pEGFP-N1 vector (Clontech) and the mutants (ΔLBD, ΔDimer, D327W/F349L, P681H) were generated by site-directed mutagenesis. .. HEK-Blue Null1 and HEK-Blue hTLR2 cells (5.4 × 104 cells per well) were seeded into the 96-well plate containing reagents in a volume of 180 μl per well and incubated in HEK-Blue Detection medium (InvivoGen) at 37 ◦C for 16 h. After 20 h, secreted embryonic alkaline phosphatase (SEAP) levels were determined using a spectrophotometer at 620 nm.

    Article Title: PLK2 disrupts autophagic flux to promote SNCA/α-synuclein pathology.
    Article Snippet: .. EGFP-DCTN1 was generated by excising full-length DCTN1 cDNA from myc-DCTN1 [72] and inserting it into the pEGFP-N1 vector (Clontech, 6085-1) at the HindIII and EcoRI sites. .. Point mutants of EGFP-SNCA [84], Flag-PLK2 (Youbio, G121393) and EGFP-DCTN1 were generated via site-directed mutagenesis via a MutanBEST kit (Takara, R401) with the primers listed in Table S1.

    Mutagenesis:

    Article Title: TLR2 immunotherapy suppresses neuroinflammation, tau spread, and memory loss in rTg4510 mice.
    Article Snippet: .. Human TLR2 was subcloned into pEGFP-N1 vector (Clontech) and the mutants (ΔLBD, ΔDimer, D327W/F349L, P681H) were generated by site-directed mutagenesis. .. HEK-Blue Null1 and HEK-Blue hTLR2 cells (5.4 × 104 cells per well) were seeded into the 96-well plate containing reagents in a volume of 180 μl per well and incubated in HEK-Blue Detection medium (InvivoGen) at 37 ◦C for 16 h. After 20 h, secreted embryonic alkaline phosphatase (SEAP) levels were determined using a spectrophotometer at 620 nm.

    Modification:

    Article Title: Determination of metal ion transport rate of human ZIP4 using stable zinc isotopes.
    Article Snippet: The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. .. The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus. ..



    Similar Products

    99
    Vazyme Biotech Co pegfp n1 vector
    Pegfp N1 Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/ClonExpress+II+One+Step+Cloning+Kit/pm40482876-75-27-41
    Average 99 stars, based on 1 article reviews
    pegfp n1 vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    TaKaRa pegfp n1 vector between sali
    Pegfp N1 Vector Between Sali, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/Sal+I/pmc11494317__pnas__2402674121__sapp-233-9-13
    Average 96 stars, based on 1 article reviews
    pegfp n1 vector between sali - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    95
    TaKaRa pegfp n1 plasmid
    Pegfp N1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/%E2%97%8ApHEK293+Ultra+Expression+Vector+I/pm41899473-72-5-8
    Average 95 stars, based on 1 article reviews
    pegfp n1 plasmid - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    93
    Addgene inc pegfp n1 vector
    Pegfp N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/pcDNA3%2E1+puro+Nodamura+B2+(Plasmid+%2317228)/pmc12628023-87-13-16
    Average 93 stars, based on 1 article reviews
    pegfp n1 vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    TaKaRa pegfp n1
    Pegfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/pmCherry-N1+Vector/pmc12370622-28-90-91
    Average 96 stars, based on 1 article reviews
    pegfp n1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    TaKaRa pegfp n1 in pcdna3 0
    Pegfp N1 In Pcdna3 0, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/pGFP+Vector/pmc12141203-1-0-6
    Average 94 stars, based on 1 article reviews
    pegfp n1 in pcdna3 0 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    93
    Addgene inc phiv egfp lentiviral vector addgene
    Phiv Egfp Lentiviral Vector Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/pEGFP-N1-TFE3+(Plasmid+%2338120)/pm40222010-746-229-232
    Average 93 stars, based on 1 article reviews
    phiv egfp lentiviral vector addgene - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    TaKaRa pegfp n1 mammalian expression vector
    Pegfp N1 Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/Nhe+I/bio_rxiv__2025__02__10__637577-293-27-32
    Average 96 stars, based on 1 article reviews
    pegfp n1 mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results